Journal: Viruses

Article Title: Small-Molecule RAF265 as an Antiviral Therapy Acts against PEDV Infection

doi: 10.3390/v14102261

Figure Lengend Snippet: RAF265 suppressed the synthesis of viral proteins of PEDV. ( A ) Vero-WT and Vero-eIF4E (S209A) cells were challenged with PEDV for different times; cells lysates were harvested for WB analysis with indicated antibodies. ( B ) Vero-WT and Vero-eIF4E (S209A) cells were infected with PEDV in the presence of increasing doses of RAF265 for 24 h. Two cell lines were harvested for WB analysis with indicated antibodies. ( C ) Schematic representation of dual-targeting inhibitor RAF265 as active antiviral. After binding, PEDV enters into Vero cells by endocytosis, RAF265 mediates arrangement of actin cytoskeleton involved in the internalization of viral infection of PEDV. RAF265 also decreases the level of p-eIF4E to inhibit the synthesis of viral proteins. h.p.i, hours post-infection; MOI, multiplicity of infection.

Article Snippet: Rabbit polyclonal antibody to eIF4E (1:1000) was purchased from Proteintech (Wuhan, China).

Techniques: Infection, Binding Assay